Scott Johnson
Production Manager Biological Process and Development Facility University of Nebraska-Lincoln
Contact
- Address
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OTHM B368D
Lincoln NE 68583-0668 - Phone
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402-472-8659 On-campus 2-8659
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scott.johnson@unl.edu
Scott Johnson joined the Biological Process and Development Facility in 1997. As the GMP Coordinator, he oversees production and process development activities for both upstream and downstream activities. He has a broad background in microbial fermentation and protein purification as well as GMP production scale up. Mr. Johnson’s work has involved process development of high cell density microbial fermentations producing recombinant proteins, harvest method using disc-stack centrifugation, tangential flow filtration, cell disruption, column chromatography, enzymatic digestions and other chemical modifications of biomolecules. He holds a MS in Biological Chemistry and a BS in Biology from the University of Nebraska.
List of Publications
Cerini F, Gaertner H, Madden K, Tolstorukov I, Brown S, Laukens B, Callewaert N, Harner JC, Oommen AM, Harms JT, Sump AR, Sealock RC, Peterson DJ, Johnson SK, Abramson SA, Meagher MM, Offord R, Hartley O, 2015, “A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris,” Protein Expression and Purification, 119, 1-10.
Veldkamp CT, Koplinski CA, Jensen DR, Peterson, FC, Smits KM, Smith BL, Johnson SK, Lettieri C, Buchholz WG, Solheim JC, Volkman BF, 2015, “Chemokine refolding and quality control measures,” Methods in Enzymology, submitted.
Meagher MM, Seravalli JG, Swanson ST, Ladd RG, Pal Khansa Y, Inan M, Harner JC, Johnson SK, Van Cott K, Lindsey C, Wannemacher R, Smith LA, 2011, “Process development and cGMP manufacturing of a recombinant ricin vaccine: An effective and stable recombinant ricin a-chain vaccine-RVEcÔ,” Biotechnol Progress 27(4), 1036-1047.
Johnson SK, Zhang W, Smith LA, Hywood-Potter KJ, Todd Swanson S, Schlegel VL, Meagher MM, 2003, “Scale-up of the fermentation and purification of the recombinant heavy chain fragment C of botulinum neurotoxin serotype F, expressed in Pichia pastoris,” Protein Expression and Purification 32(1), 1-9.
Johnson, SK, Bhattacharyya, S, and Griep, MA, 2000, “DnaB Helicase Stimulates Primer Synthesis Activity on Short Oligonucleotide Templates,” Biochemistry 39, 736-744.
Griep, MA, Adkins, BJ, Hromas, D, Johnson, SK, and Miller, J, 1997, “The Tyrosine Photophysics of a Primase-Derived Peptide Are Sensitive to the Peptide's Zinc-Bound State: Proof that the Bacterial Primase Hypothetical Zinc Finger Sequence Binds Zinc,” Biochemistry 36, 544-553.